Retinal neovascularisation without ischaemia in the spontaneously diabetic Torii rat
作者： H. Yamada , E. Yamada , A. Higuchi , M. Matsumura
DOI: 10.1007/S00125-005-1809-0
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摘要: The spontaneously diabetic Torii (SDT) rat has recently been established as a model of type 2 human diabetes mellitus. Male SDT rats develop severe ocular complications. This study investigated the nature complications in this and addressed question whether is good proliferative retinopathy. aged 50 weeks were studied for period 8 months. Under deep anaesthesia, one eye each animal was enucleated following perfusion with fluorescein dextran retinal flat mount prepared to vascular structure. other histologically by haematoxylin–eosin azan staining immunohistochemistry using antibodies against endothelium (Griffonia simplicifolia isolectin B4 antibody) endothelial growth factor (VEGF). From structure study, 17 32 (53%) showed retinopathy without non-perfusion. histological revealed traction folds Azan some matrix normal those compared normoglycaemic controls. Staining Griffonia antibody no specific changes any rats, while VEGF higher immunoreactivity retina retinopathy, but only low control animals. There appear be differences between develops neovascularisation ischaemia. very unique display may caused increased expression VEGF.
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