Immunochemical demonstration of the clonal organization of chimaeric mouse epidermis.

摘要: The clonal organization of chimaeric mouse epidermis was demonstrated by in situ staining whole-mount preparations using monoclonal antibodies directed against H-2k and H-2b antigens. A striking pattern transversely oriented stripes or patches found which extended from mid-dorsum to the flank region. orientation these indicates a preferred directional expansion clones during development dorsal/lateral epidermis. belly region differed that were not found, but marked ventral midline boundary observed. This demarcation line may be due physical effect, i.e. isolation left right halves early embryogenesis with relatively little cell mingling following closure abdominal wall. obvious nonhomogenous distribution components contradicts assumptions homogenous, fine-grained patchiness derived electrophoretic analysis tissue samples used studies skin carcinogenesis. observation hair follicles contain cells both parental genotypes implies polyclonal origin. Epidermal proliferative units as described Potten (1974) revealed mosaicism at cellular level sheets. compartment each putative epidermal unit is polyclonal.