New Method for the Isolation and Identification of Methanogenic Bacteria

作者: T. Edwards , B. C. McBride

DOI: 10.1128/AM.29.4.540-545.1975

关键词:

摘要: A new technique is reported for the rapid growth and detection of methanogenic bacteria by using petri plates. The method employs an anaerobic glove box containing inner chamber with separate gas-flushing facilities. numbers recovered from domestic sewage sludge are comparable to those other methods. organisms isolated include Methanosarcina, Methanospirillum, Methanobacterium strain M.o.H., formicicum. Identification colonies facilitated taking advantage unique fluorescence properties these organisms. Colonies as small 0.5 mm can be detected exposing them long-wave ultraviolet light.

参考文章(6)
R.S. Wolfe, Microbial Formation of Methane Advances in Microbial Physiology. ,vol. 6, pp. 107- 146 ,(1971) , 10.1016/S0065-2911(08)60068-5
M. P. Bryant, B. C. McBride, R. S. Wolfe, Hydrogen-oxidizing Methane Bacteria I. Cultivation and Methanogenesis Journal of Bacteriology. ,vol. 95, pp. 1118- 1123 ,(1968) , 10.1128/JB.95.3.1118-1123.1968
E.A. Wolin, M.J. Wolin, R.S. Wolfe, Formation of Methane by Bacterial Extracts Journal of Biological Chemistry. ,vol. 238, pp. 2882- 2886 ,(1963) , 10.1016/S0021-9258(18)67912-8
P. Cheeseman, A. Toms-Wood, R. S. Wolfe, Isolation and Properties of a Fluorescent Compound, Factor420, from Methanobacterium Strain M.o.H Journal of Bacteriology. ,vol. 112, pp. 527- 531 ,(1972) , 10.1128/JB.112.1.527-531.1972
Alexander Aranki, Rolf Freter, Use of anaerobic glove boxes for the cultivation of strictly anaerobic bacteria The American Journal of Clinical Nutrition. ,vol. 25, pp. 1329- 1334 ,(1972) , 10.1093/AJCN/25.12.1329
R. E. Hungate, THE ANAEROBIC MESOPHILIC CELLULOLYTIC BACTERIA 1 Bacteriological Reviews. ,vol. 14, pp. 1- 49 ,(1950) , 10.1128/BR.14.1.1-49.1950