Method for producing a recombinant protein using a cell line adapted to a protein-free and lipid-free medium

作者: Akiyoshi Taniguchi , Tetsuji Sasaki

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摘要: A method for producing a recombinant protein includes steps of: (a) culturing transformed cell in protein-free and lipid-free medium containing no exogenous growth factors, which the is produced by transfecting of line derived from Chinese Hamster Ovary (CHO) cells, adapted to medium, capable proliferating suspended state with vector gene coding be under control promoter operable cell, (b) recovering cell.

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