N-acetylneuraminyllactose-binding fibrillar hemagglutinin of Campylobacter pylori: a putative colonization factor antigen.

摘要: Campylobacter pylori is the causative agent of gastritis and possibly peptic duodenal ulcers in adults. Histological observations show C. attached to gastric epithelium as well mucus layer stomach. We found that clinical isolates possess a cell-bound hemagglutinin detectable with human erythrocytes (all phenotypes tested) those variety animal species. The antigenic, heat sensitive, destroyed by pronase papain but resistant pepsin trypsin. has fibrillar morphology; pylori-erythrocyte interaction displays very intimate contact, which typical fibrillae-mediated attachment. Fibrillae were removed from solubilization N-octylglucose. After partial purification removal N-octylglucose dialysis, protein reaggregated, assembly structures. Hemagglutination inhibition was observed sialoproteins fetuin, alpha 2-macroglobulin, glycophorin A not asialofetuin or asialoglycophorin A. erythrocyte receptor more sensitive destruction neuraminidase specific for N-acetylneuraminyl-alpha(2-3)-galactopyranosyl [NeuAc(2-3)Gal] sequence than one NeuAc(2-6)Gal. Hemagglutination-inhibition assays N-acetylneuraminyl-alpha(2-3)-lactose [NeuAc(2-3)-lactose] NeuAc(2-6)-lactose confirmed preferentially binds NeuAc(2-3)Gal isomer NeuAc-lactose. Based upon above-described properties hemagglutinin, we conclude this antigen should be designated putative colonization factor antigen. Images