Preservative solution that stabilizes erythrocyte morphology and leukocyte viability under ambient conditions

摘要: The deterioration of whole blood ex vivo represents a logistical hurdle in clinical and research settings. Here, cocktail preservative is described that stabilizes leukocyte viability erythrocyte morphology under ambient storage. Neutrophil biostabilization was explored using sophisticated microfluidic assay to examine the effectiveness caspase inhibition stabilize purified neutrophils. Following 72 h storage, neutrophils remained fully functional migrate towards chemical cues maintained their ability undergo NETosis after stimulation. Furthermore, stored exhibited improved CD45 biomarker retention and reduced apoptosis mortality compared untreated controls. To morphology, solution formulated Taguchi methods experimental design, combined with inhibitor form solution, CSWB. CSWB evaluated from healthy donors women metastatic breast cancer conditions for 72 h. CSWB-treated samples showed significant improvement Leukocytes significantly higher This shelf life an opportunity transport isolates or simply ease timelines where degradation problematic.