Cytology, immunopathology and flow cytometry in the diagnosis of pleural and peritoneal effusions.

摘要: There were 106 pleural and peritoneal effusions studied in order to investigate the contribution of immunocytochemistry flow cytometry routine cytologic diagnosis. A panel antibodies (to cytokeratin, vimentin, human milk fat globule, epithelial membrane antigen carcinoembryonic antigen) was applied aceton-fixed slides, using immunoperoxydase immunofluorescence methods. Flow performed a double labeling method, i.e., propidium iodide for DNA staining keratin cells. In this way aneuploidy more evident histograms when fluid contained many reactive nonepithelial cells (lymphocytes). designation marker profiles made three most frequently occurring diagnoses, mesothelial proliferation (I), adenocarcinoma (II), malignant mesothelioma (III). For differentiation between mesothelioma, useful as 75% adenocarcinomas antigen-positive mesotheliomas consistently negative. Furthermore, DNA-aneuploidy strongly supported diagnosis adenocarcinoma, DNA-euploid, even though occasional found different from same patient examined. globule and/or antigen, study proved be reliable, their presence indicating malignancy. It is stressed that method used (fixation, antibodies, washing procedures) can influence these findings. 16 patients (17%) performing immunopathology meant an important