Sequence-characterized amplified regions that differentiate New World screwworms from other potential wound-inhabiting flies:

摘要: New World screwworms, Cochliomyia hominivorax (Coquerel, 1858), were once devastating pests of warm-blooded animals in the United States before they successfully eradicated using sterile insect technique. Guarding against introduction screwworms to North America or any other screwworm-free area relies on rapid, reliable identification suspected cases. In current study, DNA from excised markers generated by randomly amplified polymorphic polymerase chain reaction was used as basis generate 2 species-specific sequence-characterized region molecular markers. Resulting primer pairs, named CR92A1 and J1A2 (each with forward reverse components), produced amplicons 852 848 base respectively. The pairs discriminated between C. hominivorax, macellaria (Fabricius, 1775), 8 species blowflies, 3 noncalliphorid dipterans, 1 nondipteran outlier. These primers may become important tools for veterinary laborat...