Cortical relay neurons and interneurons in the N. ventralis posterolateralis of cats: A horseradish peroxidase, electron-microscopic, golgi and immunocytochemical study

摘要: Abstract After injections of horseradish peroxidase involving the whole primary (SI) and secondary somatosensory (SII) areas adult cats, 16–21% out 2220 counted neurons in nucleus ventralis posterolateralis were unlabelled. The mean perikarya these varied between111.8 ± 32.3 μm 2 and180.8 48.6μm . size retrogradely-labelled neuron ranged from256.9 100.4μm to409 163 Retrogradely-labelled unlabelled examined under light- high-voltage electron-microscopy. Besides ‘large’, mainly multipolar or oval fusiform perikarya, may display ‘small’ size. Both types correlate well with Golgi-impregnated cells a tufted dendritic pattern usually identified as thalamocortical neurons. On other hand, morphology initial dendrites by retrograde transport that which are markedly different from neurons, have very characteristic profuse appendages been previous investigators Golgi Type II In order to probe further whether correspond GABAergic interneurons proposed evidence, an immunocytochemical approach was also applied at electron-microscope level, using antiserum prepared sheep against rat brain glutamate decar☐ylase. By this method it is shown 19–21% cats decar☐ylase-positive perikaryal labelled ranges between134.6 44.5μm and164.4 47.3μm Histogram distribution number immunoreactive closely matches experiments peroxidase. results give support evidence suggesting part population represented distinct class apparently GABAergic.