Cloning of mce1 locus of Mycobacterium leprae in Mycobacterium smegmatis mc2 155 SMR5 and evaluation of expression of mce1 genes in M. smegmatis and M. leprae
作者： Ramachandran Sarojini Santhosh , Shunmugiah Karutha Pandian , Nirmala Lini , Abdul Khader Shabaana , Avuthu Nagavardhini
DOI: 10.1016/J.FEMSIM.2005.05.004
关键词:
摘要: Plasmid pSET152 is a broad host range mobilizable vector which integrates into streptomyces chromosome utilizing att site and int function of slashed circleC31. Transformation this plasmid Mycobacterium smegmatis mc2 155 SMR5 gave stable transformants carrying the as an integrated copy. Integration occurred at cross over sequence 5'TTG disrupting gatA gene (Glu-tRNA(Gln) amidotransferase subunitA), non-essential under conditions used. Recombinant plasmids mce1 locus leprae were used to construct M. in their chromosome. RT-PCR analysis revealed specific transcripts mce smegmatis. The transcribed mRNA carried intergenic regions between genes indicating that operon. Examination from lepromatous leprosy patient's biopsy showed operon pathogen also.
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