11 Electron Microscopy of Isolated Microbial Membranes

摘要: Publisher Summary This chapter describes the electron microscopy of isolated microbial membranes. For isolation membranes it is usually necessary to break up individual cells because only outer surface layers and membrane bacterial envelope are assailable from outside. The cytoplasmic membrane, which surrounds protoplast, intracytoplasmic like photosynthetic bacteria accessible after removal a more or less rigid cell by one mechanical chemical methods. principle methods described in disintegration processes. power can be obtained different methods—(1) shaking together with glass beads aluminum powder special apparatus; (2) use pressure, for example French pressure cell, sonication sonifier. A variety have been developed avoid using strength, instead lytic enzymes degrading chemicals isolation. One first used most powerful lysozyme, attacks very specifically peptidoglycan walls.