Pre-analytical Requirements for Analyzing Nucleic Acids from Blood

摘要: Circulating nucleic acids have received an increasing scrutiny over the past decade with some applications, such as in prenatal diagnosis and oncology, being on verge of use clinical practice. It is crucial to implement optimal standardization pre-analytical procedures. Currently, this domain has been poorly studied there no well-established procedure. This chapter examines literature factors affecting from blood drawing storage circulating cell-free DNA extracts ready for analysis provides elements guidelines a set In particular, reports choice between serum plasma biological source but does not concern actual acid extraction procedures (these will be dealt “ miRNA Isolation”). lack standard operating procedure application handling setting due dispensing sharing data among researchers well head-to-head comparative studies techniques. led in-house specific that are, undoubtedly, prejudicial smooth translation into Hence, proposed should overcome gap technique.