Functional analysis of the cfdp1 gene in zebrafish provides evidence for its crucial role in craniofacial development and osteogenesis

摘要: The CFDP1 proteins have been linked to craniofacial development and osteogenesis in vertebrates, though specific human syndromes not yet identified. Alterations of represent the main cause infant disability mortality humans. For this reason, it is crucial understand cellular functions mechanism action protein model vertebrate organisms. Using a combination genomic, molecular cell biology approaches, we performed functional analysis cfdp1 gene its encoded protein, zCFDP1, zebrafish system. We found that zCFDP1 present zygote, rapidly produced after MTZ transition highly abundant head structures. Depletion induced by an ATG-blocking morpholino, produces considerable defects structures bone mineralization. Together, our results show essential required for proper provide first experimental evidence showing vertebrates actively participates morphogenesis territories.