Progesterone secretion by induced corpora lutea (CL) of anestrous bitches after administration of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (HOG).

摘要: Sixteen bitches were treated with pregnant mare serum gonadotropin (PMSG; 44 IU/kg bwt) intramuscularly (i.m.) for nine consecutive days and each was given 500 IU HOG i.m. on the tenth day or first of induced estrus (Day 0). On Day 12, bitch randomly assigned to three groups as follows: Group 1-six bitches, 10 mcg GnRH i.m.once every 24 hr; 2-six once 3-four 5 ml 0.9% saline hr. Treatments continued until 55. Blood samples obtained prior treatment other Plasma progesterone concentrations determined by radioimmunoassay (RIA) data arranged using a split-plot design, main plot sampling subplots. Analysis Duncan's Multiple Range Test treatment-by-day interaction least-squares method. After treatment, from 2 higher (P<0.05) than those groups. A significant effect seen 20 (P<0.01), while 50 difference approached significance. These findings suggest that is capable stimulating production CL in bitch. However, neither nor prevented premature regression these CL. Results this study luteolysis probably not due lack gonadotrophic support either hypothalamus anterior pituitary gland.