Structural features, antioxidant and acetylcholinesterase inhibitory activities of polysaccharides from stem of Physalis alkekengi L.
作者： Xuegui Liu , Jun Bian , Danqi Li , Changfeng Liu , Shuangshuang Xu
DOI: 10.1016/J.INDCROP.2018.12.047
关键词:
摘要: Abstract Physalis alkekengi L. (P. alkekengi) is an important medicinal plant in China. In this study, two polysaccharides (SPAP-1 and SPAP-2) were isolated from the stem of P. using hot water extraction method. The extract was further purified by DEAE-cellulose Sephadex G-200 chromatography. chemical characteristics, surface features thermal stability SPAP-1 SPAP-2 evaluated. For first time, high-performance liquid chromatography with refractive index detection amino column (without sample derivatization) performed to analyze monosaccharide composition obtained alkekengi. As a result, (Mw = 9.1 kDa) consisted rhamnose, arabinose, galacturonic acid, mannose, glucose ratio 1.0:1.5:1.9:29.1:1.03, while (Mw = 13.5 kDa) comprised xylose, mannose 31.8:14.8:1.0:6.5:22.6. Additionally, antioxidant effects evaluated ferric reducing power, 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate acid) (ABTS) 2.2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. results showed that exerted significant activities. Subsequently, acetylcholinesterase inhibitory vitro investigated, which that, moderate Thus, polysaccharide content as promising source natural products possess good activities may exert beneficial on cognitive function.
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