Linalool and linalool oxide production in transgenic carnation flowers expressing the Clarkia breweri linalool synthase gene
作者： Michal Lavy , Amir Zuker , Efraim Lewinsohn , Olga Larkov , Uzi Ravid
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摘要: Most modern cut-flower cultivars, including those of carnation(Dianthus caryophyllus), lack distinct fragrance.Carnationcv. Eilat flowers produce and emit various fragrance compounds, includingbenzoic acid derivatives sesquiterpenes, but not monoterpenes. Based onGC-MS analysis, benzoic acid, benzyl benzoate, phenylethyl methylbenzoate, cis-3-hexenyl benzoate β-caryophylleneare the major representing ca. 60% total volatilesgenerated by these flowers. The level compounds increases dramaticallyduring petal development. To evaluate possibility producing monoterpenesin carnation cv. Eilat, we generated transgenic plants expressing linaloolsynthase gene from Clarkia breweri under regulation ofthe CaMV 35S constitutive promoter. product this catalyzes theproduction monoterpene linalool geranyl diphosphate. HeadspaceGC-MSanalysis revealed that leaves transgenic, controlplants,emit its derivatives, cis- andtrans-linalool oxide. GC-MS analysis extractrevealed accumulation trans-linalool oxide notlinalool. emission did lead todetectable changes in flower scent for human olfaction.
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sci-hub.se 参考文章(32)
Amir Zuker, Asaph Ahroni, Tzvi Tzfira, Hagit Ben-Meir, Alexander Vainstein, Wounding by bombardment yields highly efficient Agrobacterium-mediated transformation of carnation ( Dianthus caryophyllus L.) Molecular Breeding. ,vol. 5, pp. 367- 375 ,(1999) , 10.1023/A:1009671131200
Joost Lücker, Harro J. Bouwmeester, Wilfried Schwab, Jan Blaas, Linus H. W. Van Der Plas, Harrie A. Verhoeven, Expression of Clarkia S-linalool synthase in transgenic petunia plants results in the accumulation of S-linalyl-β-d-glucopyranoside The Plant Journal. ,vol. 27, pp. 315- 324 ,(2001) , 10.1046/J.1365-313X.2001.01097.X
Jensen, Merle H. Malter, Alan J., Protected Agriculture: A Global Review ,(1995)
E. Pichersky, R. A. Raguso, E. Lewinsohn, R. Croteau, Floral scent production in Clarkia (Onagraceae). I: Localization and developmental modulation of monoterpene emission and linalool synthase activity Plant Physiology. ,vol. 106, pp. 1533- 1540 ,(1994) , 10.1104/PP.106.4.1533
Jennifer S. Thaler, Jasmonate-inducible plant defences cause increased parasitism of herbivores Nature. ,vol. 399, pp. 686- 688 ,(1999) , 10.1038/21420
Harry T. Lawless, Hildegarde Heymann, Sensory Evaluation of Food Food Science Text Series. ,(2010) , 10.1007/978-1-4419-6488-5
Michael A Phillips, Rodney B Croteau, Resin-based defenses in conifers Trends in Plant Science. ,vol. 4, pp. 184- 190 ,(1999) , 10.1016/S1360-1385(99)01401-6
Kevin E. McBride, Kristin R. Summerfelt, Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Molecular Biology. ,vol. 14, pp. 269- 276 ,(1990) , 10.1007/BF00018567
S. S. Mahmoud, R. B. Croteau, Metabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 8915- 8920 ,(2001) , 10.1073/PNAS.141237298
Vincenzo De Luca, Benoit St Pierre, The cell and developmental biology of alkaloid biosynthesis Trends in Plant Science. ,vol. 5, pp. 168- 173 ,(2000) , 10.1016/S1360-1385(00)01575-2