Degradation of the endocrine-disrupting dimethyl phthalate carboxylic ester by Sphingomonas yanoikuyae DOS01 isolated from the South China Sea and the biochemical pathway

摘要: Abstract Bacteria capable of using dimethyl phthalate (DMP) as the sole carbon and energy source were isolated from sediments collected at a depth 1340 m South China Sea. Sphingomonas yanoikuyae DOS01, identified based on 16S rRNA gene sequence, utilized DMP an initial level 180 mg l −1 to non-detectable in 35 h 30 °C, optical density (OD 600 ) values increased over time incubation. Degradation intermediate monomethyl (MMP) accumulated up 21.3 mg then disappeared culture medium. When MMP or another (PA) was used substrate, this strain only degrading MMP, but not PA. Total organic (TOC) analysis medium suggested that both mineralized, This deep-ocean sediment transforms common biochemical pathway for reported before. Further esterase activity assays indicated enzyme induced by has higher affinity than substrate p -nitrophenyl acetate. Our results complete degradation marine microorganism may involve new pathway.