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摘要: TRIATOMINAE (HEMIPTERA:REDUVIIDAE) IN TEXAS: MITOCHONDRIAL GENOME ASSEMBLY, TRYPANOSOMA CRUZI DETECTION, AND BACTERIAL COMMUNITY ANALYSIS Chissa-Louise Rivaldi Thesis Chair: Blake Bextine, Ph. D. The University of Texas at Tyler May 2015 genus Triatoma contains vectors the protozoan parasite Trypanosoma cruzi, pathogen responsible for Chagas disease. following research presents several inductive studies to develop vector control approaches targeted or species level. Two-hundred and eighty-two insects were collected, identified, tested presence T. cruzi. Four collected overall incidence cruzi was 52% (T. gerstaeckeri -51%, lecticularia – 92%, other, 29%). From these 282 insects, bacterial communities eight specimens sequenced analyzed using pyrosequencing technology. assigned taxonomy in silica. Bacterial consistent with previous analyses conducted differing methods, had low alpha beta diversity, which makes this system ideal a approach. Whole genomic mitochondrial DNA isolated from two Triatoma. This mtDNA then high-thoroughput sequencing platform. resulting sequence data assembled de novo referenced an existing genome dimidiata, leading South America). mitogenomes similar, containing approximately 17 000 kbp, similar coding regions, 30% GC content, suggesting little divergence between species. A gene deletion found when compared both dimidiata gerstaeckeri, could be useful efforts. Results should used implement strategies inhibit spread