作者: Leonie Suter , Andrea Maree Polanowski , Laurence John Clarke , John Andrew Kitchener , Bruce Emerson Deagle
DOI: 10.1111/MEC.15587
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摘要: Environmental DNA (eDNA) metabarcoding is emerging as a novel, objective tool for monitoring marine metazoan biodiversity. Zooplankton biodiversity in the vast open ocean currently monitored through continuous plankton recorder (CPR) surveys, using ship‐based bulk sampling and morphological identification. We assessed whether eDNA (2 L filtered seawater) could capture similar Southern Ocean zooplankton conventional CPR (~1,500 seawater per sample). directly compared with (a) (b) of collected (two transects each comparison, 40 44 paired samples, respectively). A metazoan‐targeted cytochrome c oxidase I (COI) marker was used to characterize species‐level diversity. In 2 this amplified large amounts non‐metazoan picoplanktonic algae, but still detected up 1.6 times more species than morphologically analysed samples. COI samples mostly avoided nonmetazoan amplifications recovered metabarcoding. However, roughly two thirds identified taxa contributing community differentiation across subtropical front separating transects. observed diurnal pattern data copepods which perform diel vertical migrations, indicating surprisingly short temporal signal. Compared COI, eukaryote‐targeted 18S ribosomal RNA higher proportion, lower diversity, metazoans eDNA. With refinement standardization methodology, become an efficient