Recombinant antibodies to small analytes and prospects for deriving them from synthetic combinatorial libraries

摘要: During the past 4 years, several laboratories have developed new methods of cloning antibody combining site genes from hybridomas or B‐lymphocytes, and functionally expressing them in bacteria, yeast, mammalian cells plants. At least three research groups also constructed ‘antibody display’ libraries with vastly diverse sequence permutations bacteriophage. These semi‐synthetic combinatorial present an repertoire orders magnitude greater than that accessed by conventional hybridoma technology. They may yield antibodies not obtainable through immunization. We cloned immunoglobulin a specific for phenylurea herbicide, diuron, into phage display vector. The fragments (Fabs) were as sensitive parent monoclonal detecting free diuron competition enzyme immunoassays. derived hapten‐specific clones synthetic libraries, but only fe...