Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin.
作者： K Takei , H Stukenbrok , A Metcalf , GA Mignery , TC Sudhof
DOI: 10.1523/JNEUROSCI.12-02-00489.1992
关键词:
摘要: The nature of second messenger-responsive intracellular Ca2+ stores in neurons remains open for discussion. Here, we demonstrate the existence Purkinje cells (PCs) endoplastic reticulum (ER) subcompartments characterized by an uneven distribution three proteins involved storage and release: inositol 1,4,5-trisphosphate (InsP3) receptor, Ca(2+)-ATPase, calsequestrin. Ca(2+)-ATPase InsP3 receptor have a widespread, although not identical, throughout ER. Calsequestrin is localized smooth ER particularly concentrated pleiomorphic vesicles with moderately electron-dense core, which appear to represent subcompartment In double-labeling experiments many these were unlabeled antibodies. These results suggest key role as store possible structural basis distinct pools regulated different messengers.
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sci-hub.se 参考文章(53)
G. A. Mignery, T. C. Südhof, The ligand binding site and transduction mechanism in the inositol-1,4,5-triphosphate receptor. The EMBO Journal. ,vol. 9, pp. 3893- 3898 ,(1990) , 10.1002/J.1460-2075.1990.TB07609.X
L Fliegel, K Burns, D H MacLennan, R A Reithmeier, M Michalak, Molecular cloning of the high affinity calcium-binding protein (calreticulin) of skeletal muscle sarcoplasmic reticulum. Journal of Biological Chemistry. ,vol. 264, pp. 21522- 21528 ,(1989) , 10.1016/S0021-9258(20)88216-7
L Plessers, JA Eggermont, F Wuytack, R Casteels, A study of the organellar Ca2(+)-transport ATPase isozymes in pig cerebellar Purkinje neurons The Journal of Neuroscience. ,vol. 11, pp. 650- 656 ,(1991) , 10.1523/JNEUROSCI.11-03-00650.1991
B T Scott, H K Simmerman, J H Collins, B Nadal-Ginard, L R Jones, Complete amino acid sequence of canine cardiac calsequestrin deduced by cDNA cloning. Journal of Biological Chemistry. ,vol. 263, pp. 8958- 8964 ,(1988) , 10.1016/S0021-9258(18)68401-7
J Lytton, D H MacLennan, Molecular cloning of cDNAs from human kidney coding for two alternatively spliced products of the cardiac Ca2+-ATPase gene. Journal of Biological Chemistry. ,vol. 263, pp. 15024- 15031 ,(1988) , 10.1016/S0021-9258(18)68141-4
F Zorzato, J Fujii, K Otsu, M Phillips, N M Green, F A Lai, G Meissner, D H MacLennan, Molecular cloning of cDNA encoding human and rabbit forms of the Ca2+ release channel (ryanodine receptor) of skeletal muscle sarcoplasmic reticulum. Journal of Biological Chemistry. ,vol. 265, pp. 2244- 2256 ,(1990) , 10.1016/S0021-9258(19)39968-5
Josef Špaček, Miroslav Hartmann, Three-dimensional analysis of dendritic spines. I. Quantitative observations related to dendritic spine and synaptic morphology in cerebral and cerebellar cortices. Anatomy and Embryology. ,vol. 167, pp. 289- 310 ,(1983) , 10.1007/BF00298517
JD Lindsey, MH Ellisman, The neuronal endomembrane system. III. The origins of the axoplasmic reticulum and discrete axonal cisternae at the axon hillock The Journal of Neuroscience. ,vol. 5, pp. 3135- 3144 ,(1985) , 10.1523/JNEUROSCI.05-12-03135.1985
K Thomas, J Navarro, R J Benson, K P Campbell, R L Rotundo, R E Fine, Newly Synthesized Calsequestrin, Destined for the Sarcoplasmic Reticulum, Is Contained in Early/Intermediate Golgi-derived Clathrin-coated Vesicles Journal of Biological Chemistry. ,vol. 264, pp. 3140- 3145 ,(1989) , 10.1016/S0021-9258(18)94042-1
H.R. Pelham, Heat shock and the sorting of luminal ER proteins. The EMBO Journal. ,vol. 8, pp. 3171- 3176 ,(1989) , 10.1002/J.1460-2075.1989.TB08475.X