A Proteinaceous Gene Regulatory Thermometer in Salmonella
作者： Reini Hurme , Kurt D Berndt , Staffan J Normark , Mikael Rhen
DOI: 10.1016/S0092-8674(00)80313-X
关键词:
摘要: Novel utilization of the coiled-coil motif is presented that enables TlpA, an autoregulatory repressor protein in Salmonella, to sense temperature shifts directly and thereby modulate extent transcription repression. Salmonella cells shifted higher temperatures, such as those encountered at host entry, showed derepressed tlpA activity. tlpA::lacZ fusions indicated promoter itself insensitive thermal control was exerted by autorepressor TlpA only. In vitro studies with highly purified concentration dependence for both fully folded conformation function, indicating thermosensing based on monomer-to-coiled-coil equilibrium.
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