The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis inStreptococcus thermophilus
作者： Jason Carte , Ross T. Christopher , Justin T. Smith , Sara Olson , Rodolphe Barrangou
DOI: 10.1111/MMI.12644
关键词:
摘要: CRISPR-Cas systems are small RNA-based immune that protect prokaryotes from invaders such as viruses and plasmids. We have investigated the features biogenesis of CRISPR (cr)RNAs in Streptococcus thermophilus (Sth) strain DGCC7710, which possesses four different including representatives three major types systems. Our results indicate crRNAs each locus specifically processed into divergent crRNA species by Cas proteins (and non-coding RNAs) associated with respective locus. find Csm Type III-A Cse I-E Cas6 Cse3 (Cas6e), respectively, retain an 8-nucleotide repeat sequence tag 5' invader-targeting sequence. The also a 21-nucleotide 3' tag. two Csn II-A Sth consist 5'-truncated targeting tag; however, these distinct size between two. Moreover, Csn1 (Cas9) protein one functions production findings multiple can function independently within given organism - important consideration engineering coexisting pathways.
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sci-hub.se 参考文章(65)
Ling Deng, Roger A. Garrett, Shiraz A. Shah, Xu Peng, Qunxin She, A novel interference mechanism by a type IIIB CRISPR-Cmr module inSulfolobus Molecular Microbiology. ,vol. 87, pp. 1088- 1099 ,(2013) , 10.1111/MMI.12152
Andrew Becker, Jerard Hurwitz, The enzymatic cleavage of phosphate termini from polynucleotides. Journal of Biological Chemistry. ,vol. 242, pp. 936- 950 ,(1967) , 10.1016/S0021-9258(18)96215-0
Elizabeth Pennisi, The CRISPR Craze Science. ,vol. 341, pp. 833- 836 ,(2013) , 10.1126/SCIENCE.341.6148.833
Ido Yosef, Moran G. Goren, Udi Qimron, Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli Nucleic Acids Research. ,vol. 40, pp. 5569- 5576 ,(2012) , 10.1093/NAR/GKS216
Woong Y Hwang, Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander, Randall T Peterson, J-R Joanna Yeh, J Keith Joung, Efficient genome editing in zebrafish using a CRISPR-Cas system Nature Biotechnology. ,vol. 31, pp. 227- 229 ,(2013) , 10.1038/NBT.2501
Jason Carte, Ruiying Wang, Hong Li, Rebecca M Terns, Michael P Terns, Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes Genes & Development. ,vol. 22, pp. 3489- 3496 ,(2008) , 10.1101/GAD.1742908
Josiane E. Garneau, Marie-Ève Dupuis, Manuela Villion, Dennis A. Romero, Rodolphe Barrangou, Patrick Boyaval, Christophe Fremaux, Philippe Horvath, Alfonso H. Magadán, Sylvain Moineau, The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature. ,vol. 468, pp. 67- 71 ,(2010) , 10.1038/NATURE09523
Rachel E. Haurwitz, Martin Jinek, Blake Wiedenheft, Kaihong Zhou, Jennifer A. Doudna, Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease Science. ,vol. 329, pp. 1355- 1358 ,(2010) , 10.1126/SCIENCE.1192272
Wenyan Jiang, David Bikard, David Cox, Feng Zhang, Luciano A Marraffini, RNA-guided editing of bacterial genomes using CRISPR-Cas systems Nature Biotechnology. ,vol. 31, pp. 233- 239 ,(2013) , 10.1038/NBT.2508
Tautvydas Karvelis, Giedrius Gasiunas, Algirdas Miksys, Rodolphe Barrangou, Philippe Horvath, Virginijus Siksnys, crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus RNA Biology. ,vol. 10, pp. 841- 851 ,(2013) , 10.4161/RNA.24203