Nonviral Gene Transfer into the Skin

摘要: Corrective gene therapy requires efficient devices for the delivery of genes and sequences into target cells. In addition to viral systems which may represent system(s) choice one or another specific indication in near future, there is a considerable need alternative that can be easily produced applied, similar low-molecular substances proteins. But still an urgent improve low transfer rates have not been overcome so far with nonviral systems. For vivo application plasmid DNA has fulfill several requirements allow transcription nucleus its translation cytoplasm. Some these are common both systems, such as binding cell surface, penetration (by fusion and/or by endocytosis), release from endosome(s), nuclear membrane, nucleus, expression encoded interest via cell’s mechanisms.