Targeting renal epithelial channels for the control of insect vectors
作者： Klaus W Beyenbach , Yasong Yu , Peter M Piermarini , Jerod Denton
DOI: 10.1080/21688370.2015.1081861
关键词:
摘要: Three small molecules were identified in high throughput screens that 1) block renal inward rectifier potassium (Kir) channels of Aedes aegypti expressed HEK cells and Xenopus oocytes, 2) inhibit the secretion KCl but not NaCl isolated Malpighian tubules, after injection into hemolymph, 3) excretion vivo, 4) render mosquitoes flightless or dead within 24h. Some had swollen abdomens at death consistent with failure. VU625, most potent promising molecule for development as mosquitocide, inhibits AeKir1-mediated currents an IC50 less than 100 nM. It is highly selective AeKir1 over mammalian Kir channels, it affects only 3 68 membrane proteins. These results document failure a new mode-of-action mosquitocide development, molecular target inducing failure, promise discovery species-specific insecticides.
sci-hub.se 参考文章(82)
Klaus W. Beyenbach, Helen Skaer, Julian A.T. Dow, The Developmental, Molecular, and Transport Biology of Malpighian Tubules Annual Review of Entomology. ,vol. 55, pp. 351- 374 ,(2010) , 10.1146/ANNUREV-ENTO-112408-085512
L. Michelle Lewis, Gautam Bhave, Brian A. Chauder, Sreedatta Banerjee, Katharina A. Lornsen, Rey Redha, Katherine Fallen, Craig W. Lindsley, C. David Weaver, Jerod S. Denton, High-throughput screening reveals a small-molecule inhibitor of the renal outer medullary potassium channel and Kir7.1. Molecular Pharmacology. ,vol. 76, pp. 1094- 1103 ,(2009) , 10.1124/MOL.109.059840
Juan P. Ianowski, Michael J. O'Donnell, Robert J. Christensen, Intracellular ion activities in Malpighian tubule cells of Rhodnius prolixus: evaluation of Na+-K+-2Cl- cotransport across the basolateral membrane. The Journal of Experimental Biology. ,vol. 205, pp. 1645- 1655 ,(2002) , 10.1242/JEB.205.11.1645
V. Nene, J. R. Wortman, D. Lawson, B. Haas, C. Kodira, Z. Tu, B. Loftus, Z. Xi, K. Megy, M. Grabherr, Q. Ren, E. M. Zdobnov, N. F. Lobo, K. S. Campbell, S. E. Brown, M. F. Bonaldo, J. Zhu, S. P. Sinkins, D. G. Hogenkamp, P. Amedeo, P. Arensburger, P. W. Atkinson, S. Bidwell, J. Biedler, E. Birney, R. V. Bruggner, J. Costas, M. R. Coy, J. Crabtree, M. Crawford, B. deBruyn, D. DeCaprio, K. Eiglmeier, E. Eisenstadt, H. El-Dorry, W. M. Gelbart, S. L. Gomes, M. Hammond, L. I. Hannick, J. R. Hogan, M. H. Holmes, D. Jaffe, J. S. Johnston, R. C. Kennedy, H. Koo, S. Kravitz, E. V. Kriventseva, D. Kulp, K. LaButti, E. Lee, S. Li, D. D. Lovin, C. Mao, E. Mauceli, C. F. M. Menck, J. R. Miller, P. Montgomery, A. Mori, A. L. Nascimento, H. F. Naveira, C. Nusbaum, S. O'Leary, J. Orvis, M. Pertea, H. Quesneville, K. R. Reidenbach, Y.-H. Rogers, C. W. Roth, J. R. Schneider, M. Schatz, M. Shumway, M. Stanke, E. O. Stinson, J. M. C. Tubio, J. P. VanZee, S. Verjovski-Almeida, D. Werner, O. White, S. Wyder, Q. Zeng, Q. Zhao, Y. Zhao, C. A. Hill, A. S. Raikhel, M. B. Soares, D. L. Knudson, N. H. Lee, J. Galagan, S. L. Salzberg, I. T. Paulsen, G. Dimopoulos, F. H. Collins, B. Birren, C. M. Fraser-Liggett, D. W. Severson, Genome sequence of Aedes aegypti, a major arbovirus vector Science. ,vol. 316, pp. 1718- 1723 ,(2007) , 10.1126/SCIENCE.1138878
KW Beyenbach, DH Petzel, Diuresis in Mosquitoes: Role of a Natriuretic Factor Physiology. ,vol. 2, pp. 171- 175 ,(1987) , 10.1152/PHYSIOLOGYONLINE.1987.2.5.171
R. Masia, D. Aneshansley, W. Nagel, R. J. Nachman, K. W. Beyenbach, Voltage clamping single cells in intact Malpighian tubules of mosquitoes American Journal of Physiology-renal Physiology. ,vol. 279, pp. 747- ,(2000) , 10.1152/AJPRENAL.2000.279.4.F747
Thomas L. Pannabecker, Klaus W. Beyenbach, Wolfram Nagel, Central role of the apical membrane H+-ATPase in electrogenesis and epithelial transport in Malpighian tubules. The Journal of Experimental Biology. ,vol. 203, pp. 1459- 1468 ,(2000)
S. Terhzaz, S.A. Davies, F.C. O'Connell, J.A. Dow, J.A. Veenstra, L. Kean, V.P. Pollock, Isolation and characterization of a leucokinin-like peptide of Drosophila melanogaster The Journal of Experimental Biology. ,vol. 202, pp. 3667- 3676 ,(1999) , 10.1242/JEB.202.24.3667
J. L. Hegarty, B. Zhang, T. L. Pannabecker, D. H. Petzel, M. D. Baustian, K. W. Beyenbach, Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules. American Journal of Physiology-cell Physiology. ,vol. 261, ,(1991) , 10.1152/AJPCELL.1991.261.3.C521
S. W. Nicolson, L. C. Isaacson, Transepithelial and intracellular potentials in isolated Malpighian tubules of tenebrionid beetle. American Journal of Physiology-renal Physiology. ,vol. 252, ,(1987) , 10.1152/AJPRENAL.1987.252.4.F645