Activation of Purinergic Receptor Subtypes Modulates Odor Sensitivity

摘要: Purinergic nucleotides, including ATP and adenosine, are important neuromodulators of peripheral auditory visual sensory systems (Thorne Housley, 1996). released by the olfactory epithelium (OE) after noxious stimuli provides a physiological source for neuromodulatory substance independent efferent innervation. Here we show that multiple subtypes purinergic receptors differentially expressed in receptor neurons sustentacular support cells. Activation evoked inward currents increases intracellular calcium cultured mouse neurons. A epithelial slice preparation confocal imaging were used to measure changes response odors, (P2R) agonists, or combined odor + P2R agonists. Pharmacological studies both P2Y P2X activation exogenous endogenous significantly reduces responsiveness. Moreover, antagonists increase odor-evoked transient, providing direct evidence modulates sensitivity via Odor G-protein-coupled results increased cAMP production, opening cyclic nucleotide-gated channels, influx Ca2+ Na+, depolarization membrane, voltage- Ca2+-gated ion channels. On-cell current-clamp recordings from neonatal slices revealed reduced nucleotide-induced electrical responses. These data also idea mammalian Peripheral ATP-mediated suppression is novel mechanism during exposure olfactotoxins may be neuroprotective mechanism.