Regulation of allergic inflammation and eosinophil recruitment in mice lacking the transcription factor NFAT1: role of interleukin-4 (IL-4) and IL-5.

摘要: Transcription factors of the NFAT (nuclear factor activated T cells) family regulate expression many genes encoding immunoregulatory cytokines and cell surface proteins during immune response. The protein NFAT1 (NFATp) is expressed functional in cells, B mast natural killer cells. Here we report a detailed analysis enhanced eosinophil responses NFAT1-deficient mice, observed an vivo model allergic inflammation. In addition to pleural eosinophilia described previously, NFAT1−/− mice that have been sensitized with antigen display significant increase, relative wild-type numbers eosinophils bone marrow peripheral blood. After restimulation vitro, antigen-responsive cells from draining lymph nodes show increased mRNA Th2 interleukin-4 (IL-4), IL-5, IL-13. Consistent this finding, there pronounced increase levels IL-5 IL-13 cavities after allergen challenge vivo. Furthermore, development depends on overexpression IL-4 because it strongly inhibited by administration neutralizing antibodies either these cytokines. These results indicate are prone develop classically phenotype characterized production Thus, presence might inhibit response, perhaps interfering responses, lack or dysfunction could potentially underlie certain cases atopic disease.