Effects of Folate Deficiency on the Metastatic Potential of Murine Melanoma Cells

摘要: Experiments were designed to measure the effect of folic acid deficiency on a major determinant cancer lethality, propensity form metastases. Murine B16 melanoma cells (F10 strain) grown in folate-deficient and -supplemented media. After 3 days, deficient medium had restricted proliferative capacity, low folate levels by bioassay, increased cell volume, abnormal deoxyuridine suppression tests, accumulation S phase flow cytometry, numbers DNA strand breaks. These consistently initiated more pulmonary metastases than control when injected into host mice. Cell size did not appear be factor metastasis formation. In vitro growth rates cloning efficiencies comparable for both types as was subcutaneous tumors. We conclude that increases metastatic potential cultured cells.