Constructs and methods for the assembly of biological pathways

作者: Matthew DeLisa , Robert Conrado

DOI:

关键词:

摘要: The present invention is directed to a synthetic nucleic acid scaffold comprising one or more subunits, each subunit two different protein-binding sequences coupled together. further relates systems and methods for assembling biological pathway producing product precursor using the scaffold.

参考文章(15)
Yunfeng Lu, Ming Yan, Yang Liu, Multiple-enzyme nanocomplexes ,(2012)
Garry P. Nolan, Donald Payan, Combinatorial enzymatic complexes ,(1998)
Jay D. Keasling, John E. Dueber, Gabriel C. Wu, Ghulam Reza Khan Malmirchegini, Use of synthetic scaffolds for the production of biosynthetic pathway products ,(2009)
Kentaro Numajiri, Takahiro Yamazaki, Mayumi Kimura, Akinori Kuzuya, Makoto Komiyama, Discrete and Active Enzyme Nanoarrays on DNA Origami Scaffolds Purified by Affinity Tag Separation Journal of the American Chemical Society. ,vol. 132, pp. 9937- 9939 ,(2010) , 10.1021/JA104702Q
Joachim Müller, Christof M. Niemeyer, DNA-directed assembly of artificial multienzyme complexes. Biochemical and Biophysical Research Communications. ,vol. 377, pp. 62- 67 ,(2008) , 10.1016/J.BBRC.2008.09.078
Robert J Conrado, Jeffrey D Varner, Matthew P DeLisa, Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy. Current Opinion in Biotechnology. ,vol. 19, pp. 492- 499 ,(2008) , 10.1016/J.COPBIO.2008.07.006
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, Organization of Intracellular Reactions with Rationally Designed RNA Assemblies Science. ,vol. 333, pp. 470- 474 ,(2011) , 10.1126/SCIENCE.1206938
M. L. Bulyk, X. Huang, Y. Choo, G. M. Church, Exploring the DNA-binding specificities of zinc fingers with DNA microarrays Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 7158- 7163 ,(2001) , 10.1073/PNAS.111163698
Anna H. Chen, Pamela A. Silver, Designing biological compartmentalization Trends in Cell Biology. ,vol. 22, pp. 662- 670 ,(2012) , 10.1016/J.TCB.2012.07.002
Henri-Pierre Fierobe, Adva Mechaly, Chantal Tardif, Anne Belaich, Raphael Lamed, Yuval Shoham, Jean-Pierre Belaich, Edward A. Bayer, Design and Production of Active Cellulosome Chimeras SELECTIVE INCORPORATION OF DOCKERIN-CONTAINING ENZYMES INTO DEFINED FUNCTIONAL COMPLEXES Journal of Biological Chemistry. ,vol. 276, pp. 21257- 21261 ,(2001) , 10.1074/JBC.M102082200