Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements
作者： Hajime Ogino , Haruki Ochi , Chihiro Uchiyama , Sarah Louie , Robert M. Grainger
DOI: 10.1007/978-1-61779-992-1_15
关键词:
摘要: Identification of cis-regulatory elements, such as enhancers and promoters, is very important not only for analysis gene regulatory networks but also a tool targeted expression experiments. In this chapter, we introduce an easy reliable approach to predict interest by comparing mammalian Xenopus genome sequences, examine their activity using co-transgenesis technique in embryos. Since the bioinformatics utilizes publically available web-tools, bench biologists can easily perform it without any need special computing capability. The assay, which directly uses polymerase chain reaction (PCR) products, quickly screens candidate elements cloning-free manner.
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