Quantifying DNA concentrations using fluorometry: a comparison of fluorophores.
作者： Stephen M. Cristol , John M. Nickerson , Kalpana Rengarajan , Milan Mehta
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摘要: Detection of low concentrations DNA is important in vision research because many animal models only provide scant samples ocular tissue. Quantitative analysis double stranded now feasible using fluorometry with newer fluorophores. This technique offers a rapid way to evaluate the content based on measurement fluorescence enhancement emitted by fluorophore-bound and more sensitive than absorption spectrometry. The purpose this study was compare sensitivity several different fluorophores for measuring fluorometry. Based our studies, we conclude that SYBR Green I PicoGreen are substantially quantifying ethidium bromide Hoechst 33258.
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