Metabolomic fingerprint of Mentha rotundifolia L. Leaf tissues promotes this species as a potential candidate for sustainable production of biologically active molecules.

摘要: Background The Mentha rotundifolia L. (Lamiaceae family), is a medicinal herb used since the ancient times as an antiseptic, analgesic and anti-inflammatory agent. In present work, metabolomic profiling of two ecotypes leaf tissues spontaneously growing in North Tunisia was achieved. Methods Phenolic contents (TPC, TFC TTC) were assessed using colorimetric methods. Metabolomic extracts based on Gas Chromatography-Mass Spectrometry (GC/MS) analysis. antioxidant ability M. achieved test systems namely DPPH FRAP assays. Antimicrobial activity against set Gram negative positive bacteria estimated by measuring ID, MIC MBC values. Results Fifty metabolites identified belonging mainly to phenolics, fatty acids, terpenes, steroids aldehydes classes with qualitative quantitative variability. Most compounds are reputed bioactive potent antioxidant, antimicrobial among others effects. To confirm these findings common vitro biological activities investigated showed significant abilities both 1,1-diphenyl-2-picrylhydrazyl (DPPH) Ferric reducing potential (FRAP) Furthermore, revealed promising tested Gram+ Gram- bacterial strains (ID: 12.5-14.5 mm, MIC: 3.125-25 (µg/mL), MBC: 6.25-100 (µg/mL)). Conclusions Based our leaves source natural antioxidants diverse which could be green pharmacy, food preservation, alternative medicine therapies.