Identification of a gene cluster responsible for hydrogen evolution in Vibrio tritonius strain AM2 with transcriptional analyses
作者： Yuta Matsumura , Hidayu Al-saari , Sayaka Mino , Satoshi Nakagawa , Fumito Maruyama
DOI: 10.1016/J.IJHYDENE.2015.05.137
关键词:
摘要: Abstract Vibrio tritonius strain AM2 shows high-yield hydrogen production even under saline conditions (1.7 mol hydrogen/mol mannitol). However, the molecular mechanism of efficient has never been studied in genus Vibrio. The aim this study is to identify genes responsible for evolution V. and gene expression pattern. Complete genome analysis revealed an existence a single 24-kb cluster containing 21 genes, which are essential formation energy-conserving formate lyase (FHL) complex, be more specific vibrio FHL was structurally rather similar hyf (hydrogenase four) found Escherichia coli. Moreover, dehydrogenase (FDH-H), fhlA-type transcriptional activator hydrogenase maturation proteins (hyp) were also located downstream form “super-gene-set” complex cluster. large subunit hyfG possessed typical motifs coordinating [NiFe] center at active site, indicates able classified as [NiFe]-hydrogenase. Furthermore, that level slightly increased upon pH decrease, correlates pH-dependent tritonius. Therefore, we can conclude key enzyme acidic conditions. Moreover hyfABCDEFGHIJ-hycI-hydN-fdhF hyp could co-transcribed respectively during state. Details discussed here.
elsevier.com
sci-hub.se 参考文章(48)
P M Vignais, A Colbeau, Molecular biology of microbial hydrogenases. Current Issues in Molecular Biology. ,vol. 6, pp. 159- 188 ,(2004)
M J Axley, D A Grahame, T C Stadtman, Escherichia coli formate-hydrogen lyase. Purification and properties of the selenium-dependent formate dehydrogenase component. Journal of Biological Chemistry. ,vol. 265, pp. 18213- 18218 ,(1990) , 10.1016/S0021-9258(17)44740-5
Basem Soboh, Dietmar Linder, Reiner Hedderich, Purification and catalytic properties of a CO-oxidizing:H2-evolving enzyme complex from Carboxydothermus hydrogenoformans. FEBS Journal. ,vol. 269, pp. 5712- 5721 ,(2002) , 10.1046/J.1432-1033.2002.03282.X
S. C. Andrews, B. C. Berks, J. McClay, A. Ambler, M. A. Quail, P. Golby, J. R. Guest, A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system. Microbiology. ,vol. 143, pp. 3633- 3647 ,(1997) , 10.1099/00221287-143-11-3633
Reinhild ROSSMANN, Martin SAUTER, Friedrich LOTTSPEICH, August BOCK, Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3 requires nickel incorporation followed by C-terminal processing at Arg537 FEBS Journal. ,vol. 220, pp. 377- 384 ,(1994) , 10.1111/J.1432-1033.1994.TB18634.X
Magali Naville, Adrien Ghuillot-Gaudeffroy, Antonin Marchais, Daniel Gautheret, ARNold: A web tool for the prediction of Rho-independent transcription terminators RNA Biology. ,vol. 8, pp. 11- 13 ,(2011) , 10.4161/RNA.8.1.13346
Rajat Sapra, Marc F. J. M. Verhagen, Michael W. W. Adams, Purification and characterization of a membrane-bound hydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus. Journal of Bacteriology. ,vol. 182, pp. 3423- 3428 ,(2000) , 10.1128/JB.182.12.3423-3428.2000
K. Trchounian, A. Trchounian, Hydrogen producing activity by Escherichia coli hydrogenase 4 (hyf) depends on glucose concentration International Journal of Hydrogen Energy. ,vol. 39, pp. 16914- 16918 ,(2014) , 10.1016/J.IJHYDENE.2014.08.059
Elizabeth S. Egan, Matthew K. Waldor, Distinct Replication Requirements for the Two Vibrio cholerae Chromosomes Cell. ,vol. 114, pp. 521- 530 ,(2003) , 10.1016/S0092-8674(03)00611-1
Y NAKASHIMADA, Hydrogen production of Enterobacter aerogenes altered by extracellular and intracellular redox states International Journal of Hydrogen Energy. ,vol. 27, pp. 1399- 1405 ,(2002) , 10.1016/S0360-3199(02)00128-3