Repression of the Low Affinity Iron Transporter Gene FET4: a Novel Mechanism Against Cadmium Toxicity Orchestrated by Yap1 via Rox1

摘要: Cadmium is a well known mutagenic metal that can enter cells via nonspecific transporters, causing several cellular damages and eventually leading to death. In the yeast Saccharomyces cerevisiae, transcription factor Yap1 plays key role in regulation of genes involved stress response. We have previously shown represses expression FET4, gene encoding low affinity iron transporter able transport metals other than iron. Here, we studied relevance this repression cell tolerance cadmium. Our results indicate genomic deletion increases FET4 transcript protein levels. addition, cadmium toxicity exhibited by strain completely reversed co-deletion gene. These data correlate with increased intracellular levels observed mutant yap1. Rox1, aerobic repressor hypoxic genes, conveys Yap1-mediated FET4. further show that, scenario where activity or Rox1 compromised, activate post-transcriptional mechanisms, involving exoribonuclease Xrn1, compensate derepression thus reveal novel protection mechanism against mediated relies on impairment uptake.