Improving sensitivity in microchip electrophoresis coupled to ESI-MS/MS on the example of a cardiac drug mixture.
作者： Fabian Schwarzkopf , Tobias Scholl , Stefan Ohla , Detlev Belder
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摘要: A comprehensive study for a sensitivity optimization in MCE with mass spectrometric detection is presented. As text mixture, we chose mixture of the cardiac drugs propranolol, bisoprolol, lidocaine, procaine and studied effect different chip layouts experimental parameters aim achieving both high MS adequate electrophoretic separation. An important aspect was comparison microfluidic containing various sheath-flow channels that avoiding junctions on-chip. We utilized glass chips monolithically integrated nanospray emitter tips coupled dead volume-free to an IT spectrometer running fragmentation mode (MS(n) ). With this setup, limits down 0.6 ng/mL model compound propranolol were achieved.
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