Functional comparison of RGS9 splice isoforms in a living cell.

摘要: Two isoforms of the GTPase-activating protein, regulator G protein signaling 9 (RGS9), control such fundamental functions as vision and behavior. RGS9–1 regulates phototransduction in rods cones, RGS9–2 dopamine opioid basal ganglia. To determine their functional differences same intact cell, we replaced with mouse rods. Surprisingly, not only supported normal photoresponse recovery under moderate light conditions but also outperformed bright light. This versatility results from its ability to inactivate transducin, regardless effector interactions, whereas prefers protein-effector complex. Such makes an isoform advantageous for timely signal inactivation across a wide range stimulus strengths may explain predominant representation throughout nervous system.