Microphotometric quantitation of the reaction product of several indirect immunoperoxidase methods demonstrating monoclonal antibody binding to antigens immobilized on nitrocellulose.
作者： P H Nibbering , R van Furth
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摘要: The nitrocellulose model and microphotometry were used to investigate whether in immunoperoxidase cytochemical methods the amount of final reaction product reflects cell surface antigen. results obtained with four peroxidase methods, i.e., those using diaminobenzidine/H2O2 (DAB/H2O)2, DAB/H2O2/COCl2, DAB/H2O2/imidazole, silver intensification DAB end product, compared first. quantitative DAB/H2O2/imidazole method proved be most sensitive was selected for further studies. Cell antigens prepared by solubilization peritoneal macrophages octyl-beta-D-glucopyranoside immobilized on nitrocellulose. Monoclonal antibody binding these detected immunocytochemistry. Comparison sensitivity indirect biotin-(strept)avidin basis highest detectable dilution a lysate showed that equally sensitive. A lin...
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