Cattle gastrointestinal nematode egg-spiked faecal samples: high recovery rates using the Mini-FLOTAC technique.

摘要: BACKGROUND Faecal egg count (FEC) techniques are commonly used to detect gastrointestinal nematodes (GINs) in cattle and determine anthelmintic efficacy/resistance through the faecal reduction test (FECRT). Mini-FLOTAC is one of recommended for a standardised FEC/FECRT helminth eggs cattle. However, only paper evaluated recovery rate GIN by (compared McMaster modified-Wisconsin method) cattle, using level contamination 200 per gram (EPG) faeces collected from goat spike samples To further study faeces, this was conducted two laboratories, Belgium Italy evaluate sensitivity, accuracy, precision reproducibility (at reading levels: grids chambers) detection spiked bovine samples. METHODS In both countries, with five different levels (10, 50, 100, 500 EPG) GINs were used. The performed laboratories same expert operator standard operating procedures (SOPs) techniques. Sensitivity, accuracy calculated each technique contamination. Statistical analyses differences performance between RESULTS had higher sensitivity (100% at all EPG vs 0-66.6% chambers levels< 100 EPG), (98.1% mean value 83.2% 63.8% lower coefficient variation (10.0% 47.5% 69.4% than McMaster. There no significant difference studies Italy. CONCLUSIONS high detected similar results obtained indicated that diagnostic FEC not dependent on laboratory environment.