Replicator dominance in a eukaryotic chromosome.

摘要: Replicators are genetic elements that control initiation at an origin of DNA replication (ori). They were first identified in the yeast Saccharomyces cerevisiae as autonomously replicating sequences (ARSs) confer on a plasmid ability to replicate S phase cell cycle. The required for ARS function have been defined, but because many participate activity not components chromosomal replicators, mutational analysis ARS1 replicator located chromosome IV S. was performed. results this indicate four (A, B1, B2 and B3) either essential or important ori activation chromosome. In strain containing two closely spaced identical copies chromosome, only one is active. mechanism repression requires A element active replicator. This binding site recognition complex (ORC), putative initiator protein. process determines which used, however, depends entirely upon flanking sequences.