Wnt-activated olfactory ensheathing cells stimulate neural stem cell proliferation and neuronal differentiation
作者： Yili Yue , Qian Xue , Jiping Yang , Xiaoji Li , Zhikuan Mi
DOI: 10.1016/J.BRAINRES.2020.146726
关键词:
摘要: Olfactory ensheathing cells (OECs) are special glial localized in olfactory system which secrete large number of neurotrophic factors and promote the migration survival neurons. Canonical Wnt/β-catenin signaling is activated OECs during development facilitates growth regeneration axons. In present study, we investigated effects Wnt-activated on proliferation differentiation neural stem (NSCs) vitro. Primary neonatal mouse were cultured identified by immunostaining P75 GFAP. Wnt activation was achieved lentivirus transfection dominant-active-β-catenin (EbC) examined PY489. The conditioned medium (CM) (wOECs-CM) control (cOECs-CM) used to stimulate NSCs. assay, wOECs-CM could increase percentage Ki67/Sox2 double positive cells. Under condition, maintain expression Nestin NSCs into Tuj1-positive Taken together, our data revealed that wOECs stimulates a secretory manner, indicating combined transplantation may be beneficial for regeneration.
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