Study on the chemodrug-induced effect in nasopharyngeal carcinoma cells using laser tweezer Raman spectroscopy.

摘要: To explore the effect in nasopharyngeal carcinoma (NPC) cells after treatment with chemodrugs, Raman profiles were characterized by laser tweezer spectroscopy. Two NPC cell lines (CNE2 and C666-1) treated gemcitabine, cisplatin, paclitaxel, respectively. The high-quality spectra of without or treatments recorded at single-cell level label-free tweezers spectroscopy (LTRS) analyzed for differences alterations profiles. Tentative assignments peaks indicated that cellular specific biomolecular changes associated drug include protein structure (e.g. 1655 cm−1), DNA/RNA content 830 cm−1), destruction base pairs 785 cm−1), reduction lipids 970 cm−1). Besides, both principal components analysis (PCA) combined linear discriminant (LDA) classification regression trees (CRT) algorithms employed to further analyze classify spectral data between control group group, best accuracy 96.7% 90.0% CNE2 C666-1 This exploratory work demonstrated LTRS technology multivariate statistical has promising potential be a novel analytical strategy evaluation NPC-related chemotherapeutic drugs.