Studies on host-foodborne bacteria in intestinal three-dimensional cell culture model indicate possible mechanisms of interaction.

摘要: Spheroids of intestinal cells (Caco-2) were used to evaluate the adhesion/invasion ability Listeria monocytogenes (pathogen) and Lactobacillus sakei 1 (potential probiotic). Besides, transcriptomic analyses Caco-2 in three dimensional cultures done, with aim revealing possible host-foodborne bacteria interactions. Result adhesion assay for L. spheroids was 22.86 ± 0.33%, but it stimulated acidic pH (4.5) by presence 2% sucrose (respectively, 32.56 ± 1.35% 33.25 ± 1.26%). Conversely, invasion rate lower at 4.5, comparison non-stressed controls (18.89 ± 1.05% 58.65 ± 0.30%, respectively). adhered tridimensional cell culture (27.30 ± 2.64%), no invasiveness. There 19 21 genes down upregulated, respectively, cells, upon infection monocytogenes, which involved immunity, apoptosis; cytoprotective responses, signalling-regulatory pathways. It evidenced despite activation or deactivation several pathways counteract infection, pathogen able hijack many host defense mechanisms. On other hand, probiotic candidate correlated decreased transcription two though expression 14 others, diverse roles apoptosis, response Our data suggest use mimic epithelium is a good model gathering broad information on putative mechanisms interaction between importance food safety, can serve as basis further in-depth investigation.