P18/Stathmin1 is regulated by miR-31 in ovarian cancer in response to taxane

摘要: MicroRNAs (miRNAs) have been reported to regulate the development of chemoresistance in many tumors. Stathmin 1 (STMN1) is a microtubule-depolymerizing molecule, involved chemo-response; however, mechanism its regulation unknown. Herein, immunohistochemical study indicated significant upregulation STMN1 ovarian cancer tissues defined as resistant tumors compared with those responsive level elevated chemoresistant cells, KF-TX, parental, KF, ones. Targeting by siRNA restored taxane-sensitivity KF-TX cells. Screening miRNA profiles from KF/KF-TX cellular set followed bioinformatics-based prediction, revealed that miR-31 could be possible regulator STMN1. Down-modulation was verified quantitative RT-PCR used. Overexpression cells (KF-TX-miR-31) significantly chemo-response and reduced expression well. reduction-associated characteristics such enhanced microtubule polymerization stability, acetylated tubulin quantification, confocal visualization, G2 phase delay, were observed KF-TX-miR-31 indicating functional reduction suppressed luciferase activity reporter construct containing 3'-untranslated region (3'-UTR), confirming directly targets has therapeutic potency when introduced into cancer, combination taxane.