Production of extracellular PETase from Ideonella sakaiensis using sec-dependent signal peptides in E. coli.
作者： Hogyun Seo , Seongmin Kim , Hyeoncheol Francis Son , Hye-Young Sagong , Seongjoon Joo
DOI: 10.1016/J.BBRC.2018.11.087
关键词:
摘要: … HPLC analysis The samples were analyzed using a CMB-20A HPLC (Shimadzu), equipped with a UV/Vis detector (SPD-20A) and C18 column (SunFireTM C18, 5 μm, 4.6 × 250 mm). …
cheric.org参考文章(20)
The proteomics protocols handbook Humana Press. ,(2005) , 10.1385/1592598900
D.W. Andrews, J.C. Young, L.F. Mirels, G.J. Czarnota, The role of the N region in signal sequence and signal-anchor function. Journal of Biological Chemistry. ,vol. 267, pp. 7761- 7769 ,(1992) , 10.1016/S0021-9258(18)42580-X
Yaramah M. Zalucki, William M. Shafer, Michael P. Jennings, Directed evolution of efficient secretion in the SRP-dependent export of TolB Biochimica et Biophysica Acta. ,vol. 1808, pp. 2544- 2550 ,(2011) , 10.1016/J.BBAMEM.2011.06.004
Paolo Natale, Thomas Brüser, Arnold J.M. Driessen, Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane—Distinct translocases and mechanisms Biochimica et Biophysica Acta. ,vol. 1778, pp. 1735- 1756 ,(2008) , 10.1016/J.BBAMEM.2007.07.015
Clark F. Schierle, Mehmet Berkmen, Damon Huber, Carol Kumamoto, Dana Boyd, Jon Beckwith, The DsbA Signal Sequence Directs Efficient, Cotranslational Export of Passenger Proteins to the Escherichia coli Periplasm via the Signal Recognition Particle Pathway Journal of Bacteriology. ,vol. 185, pp. 5706- 5713 ,(2003) , 10.1128/JB.185.19.5706-5713.2003
F.J.M. Mergulhão, D.K. Summers, G.A. Monteiro, Recombinant protein secretion in Escherichia coli. Biotechnology Advances. ,vol. 23, pp. 177- 202 ,(2005) , 10.1016/J.BIOTECHADV.2004.11.003
Thomas Nordahl Petersen, Søren Brunak, Gunnar von Heijne, Henrik Nielsen, SignalP 4.0: discriminating signal peptides from transmembrane regions Nature Methods. ,vol. 8, pp. 785- 786 ,(2011) , 10.1038/NMETH.1701
J. H. Choi, S. Y. Lee, Secretory and extracellular production of recombinant proteins using Escherichia coli Applied Microbiology and Biotechnology. ,vol. 64, pp. 625- 635 ,(2004) , 10.1007/S00253-004-1559-9
Pantelis G. Bagos, Elisanthi P. Nikolaou, Theodore D. Liakopoulos, Konstantinos D. Tsirigos, Combined prediction of Tat and Sec signal peptides with hidden Markov models Bioinformatics. ,vol. 26, pp. 2811- 2817 ,(2010) , 10.1093/BIOINFORMATICS/BTQ530
Ario de Marco, Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli. Microbial Cell Factories. ,vol. 8, pp. 26- 26 ,(2009) , 10.1186/1475-2859-8-26