Neuronal and non-neuronal cell populations of the avian dorsal root ganglia express muscarinic acetylcholine receptors

摘要: The distribution of muscarinic acetylcholine receptors was investigated by immuno-light and electron microscopy in the chick dorsal root ganglion during embryonic development (E12 E18) after hatching. monoclonal antibody we used recognizes binding site shared all five receptor subtypes. At E12, light reveals several immunopositive neurons with variable degrees immunolabeling, heterogeneously distributed throughout ganglion. Later hatching, intensity immunolabeling seems to decrease neurons, small-medium-sized type, are located mostly medio-dorsal region Under microscope, immunoreaction is associated Nissl bodies, budding Golgi cisterns and, especially at discrete loci along neuronal plasma membrane. Unmyelinated nerve fibers, both central peripheral branches, also immunopositive, suggesting that transported towards spinal cord periphery, respectively. A large number perineuronal satellite cells myelinating unmyelinating Schwann intensely labeled. These observations, combined previous data on pharmacological functional characterization avian ganglion, suggest sensory non-neuronal able respond stimuli. Since receptor-immunoreactivity restricted their unmyelinated nociceptive these involved modulating transduction noxious stimuli from periphery.