Protein quantification methods to determine protein concentration prior to electrophoresis.
作者： J. P. Dean Goldring
DOI: 10.1007/978-1-61779-821-4_3
关键词:
摘要: During each step of a protein isolation technique, if enzyme activity is to be determined and before mixture separated on polyacrylamide electrophoresis gel, it important determine the concentration protein(s) in solution. Measuring involves absorbance UV range or staining with dyes copper. This review describes various determination methods that can employed measure
springer.com
springerlink.com参考文章(7)
Gerald D. Fasman, Practical Handbook of Biochemistry and Molecular Biology ,(1989)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
William E. Groves, Francis C. Davis, Bruce H. Sells, Spectrophotometric determination of microgram quantities of protein without nucleic acid interference Analytical Biochemistry. ,vol. 22, pp. 195- 210 ,(1968) , 10.1016/0003-2697(68)90307-2
P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, D.C. Klenk, Measurement of protein using bicinchoninic acid Analytical Biochemistry. ,vol. 150, pp. 76- 85 ,(1985) , 10.1016/0003-2697(85)90442-7
Steve J. Compton, Clive G. Jones, Mechanism of dye response and interference in the Bradford protein assay Analytical Biochemistry. ,vol. 151, pp. 369- 374 ,(1985) , 10.1016/0003-2697(85)90190-3
MM Bradford, None, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry. ,vol. 72, pp. 248- 254 ,(1976) , 10.1016/0003-2697(76)90527-3
Christa M. Stoscheck, Quantitation of protein. Methods in Enzymology. ,vol. 182, pp. 50- 68 ,(1990) , 10.1016/0076-6879(90)82008-P