Estimation of fluorescence yields for 2′,7′-dichlorofluorescein in titanium dioxide suspensions

摘要: Abstract A recently published computational procedure for extracting quantum yields of fluorescence, generated within a highly scattering medium, has been tested with the dye 2′,7′-dichlorofluorescein in TiO 2 suspensions. The computation is based on solution fluorescence transport under Kubelka—Munk conditions, which implies diffuse incidence exciting light, but parameters entering into it were derived from transmitted and back-scattered light measurements made more realistic conditions collimated or focused incident light. corrects reabsorption converts observed spectra suspensions reasonable approximations to spectrum dianion solution. Fluorescence alkaline approach that measured Remaining differences can probably be accounted as errors induced by polarization, larger than anticipated 450 – 500 nm wavelength range In weakly acid suspensions, adsorbed , its largely quenched; remains ascribed free dianion. It concluded procedure, determined prescribed ways, value treating systems even when are not strictly observed, especially if care taken minimize polarization.