Differentiation of Two Types of Basophils In The Adenohypophysis of The Rat and The Mouse

摘要: Pituitaries are fixed for 24 hr. in Bouin's fluid containing 0.5% trichloroacetic acid instead of 5% acetic acid, or a mixture 9 parts SUSA and 1 part saturated aqueous solution picric acid. They embedded paraffin horizontal sections cut at 3–4 μ. The staining method consists 3 phases: (a) immersion aldehyde-fuchsin the selective demonstration beta cell granules, (b) nuclei with Ehrlich's hematoxylin (c) rapid one-step counterstain light green orange G dissolved phosphotungstic-acetic differentiation acidophilic delta granules.