Interspecific Distinguishability of Veiled Lady Mushrooms (Dictyophora spp.) Based on rDNA-ITS Analysis
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DOI: 10.4489/KJM.2004.32.1.001
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摘要: To establish the phylogenetic relationships of Dictyophora spp., rDNA-ITS regions 11 strains veiled lady mushroom collected from various countries were amplified and sequenced. It was observed that divided into four groups based on PCR band patterns each ITS region cleaved by eight different restriction enzymes in polymorphic sequence analysis (CAPS). The phylogenic relationship group (CAPS) matches well with previously reported morphological phylogeny, such as 5 D. indusiata, 4 echinovolvata, a strain Phallus rugulosus. Sequence using cluster V methods showed more detail classification than CAPS analysis. 5.8S two point nucleotide base exchanges G to A according groups, subdivided variation I II regions. But rugulosus not full region. This study further delineates taxonomic level at which sequences, comparison ribosomal gene sequence, are most useful systematics other study.
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