Micropattern-mediated apical guidance accelerates epithelial cell migration to improve healing around percutaneous gastrostomy tubes

摘要: Hypergranulation, bacterial infection, and device dislodgment are common complications associated with percutaneous gastronomy (PG) tube placement for enteral feeding largely attributable to delayed stoma tract maturation around the device. Stoma is a wound-healing process that requires collective complete migration of an advancing epithelial layer. While it widely accepted micropatterned surfaces enhance cell when cells cultured directly on substrate, few studies have investigated influence apical contact guidance from migration, as occurs during formation. Here, we developed 2D 3D in vitro assays test effect various Sharklet micropatterns apically-guided migration. The modified scratch wound assay identified micropattern (+10SK50x50) enhanced by 4-fold (p = 0.0105) compared smooth controls over course seven days. best-performing was then applied cylindrical prototypes same outer diameter pediatric PG tube. These were evaluated novel where magnetic levitation aggregated create artificial stoma. Results indicated 50% increase (p<0.0001) after days along Sharklet-micropatterned controls. both assays. data suggest incorporation onto surface may accelerate via contact, improve maturation, reduce skin-associated complications.